Diadenosine polyphosphates are a family of dinucleotides with relevant properties in the eye and in other tissues. Diadenosine polyphosphates can activate P2Y and P2X receptors present on the ocular surface, anterior segment and retina. In the cornea, the presence of a P2Y2, P2Y4 and P2Y6 receptor has been identified. Both diadenosine polyphosphates and other purinergic agonists modified corneal wound healing depending on the receptor that is activated by these substances. To confirm the involvement of the P2Y2 receptor in the wound healing process after the challenge with Ap4A, we have designed siRNA against P2Y2 receptor. We have observed that P2Y2 is localized in the most external layer of the corneal epithelium. The pre-treatment with siRNA produced a disappearance of the receptor at 12 and 24 hours after the wound, being the location for P2Y2 restored 36 hours after the wound. We have also observed that in half of the tested corneas, there was an increase in the P2Y2 expression after silencing compared to control and Ap4A treated corneas, being this receptor localized both in corneal epithelium and stroma.